<- Home <- Arhive <- Vol. 21, Issue 4, December 2013

Rom J Leg Med21(4)253-258(2013)
© Romanian Society of Legal Medicine

Expression of antigens related to hypoxia, stress, and apoptosis in the myocardium after fatal carbon monoxide exposure

S. Morita, S. Furukawa, L. Wingenfeld, K. Nishi

Abstract: Although it has been described and generally accepted that the myocardium does not show any ischemic types of changes or other lesions in fatal carbon monoxide poisoning victims, we previously observed detailed pathological findings with careful staining using HE and/or Azan. We have used immnohistochemistry on the myocardium from twenty victims of both carbon monoxide poisoning and house fire. We especially selected antibodies which were previously recognized as cold stress antigens. And we also used antibodies related to stress, apoptosis, and vascular genesis. The nuclei showed good reactivity with CIRBP, RBM3, and SIRT1 antibodies. Although CIRBP and RBM3 were previously recognized as cold stress antigens, this study indicated that these antigens were also transcribed in hypoxia. Antigens related to stress, apoptosis, and vascular genesis were also detected in the myocardium from carbon monoxide poisoning victims. The results obtained in the present study suggest that antigens, such as CIRBP, RBM3 and SIRT1, may be useful temporal markers of global hypoxia or ischemia in myocardium. This study also found that the cells once exposed to stress and hypoxia, develop and activate signal pathways to prevent harmful consequences, and the expressed antigens may be retained after cell death and during the postmortem interval.
Keywords: Carbon monoxide poisoning, Hypoxic cardiac cells, CIRBP, RBM3, SIRT1.

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