DNA EXAMINATION OF MIXED TRACES ON PHYSICAL EVIDENCE, IN KINSHIP AND RECOGNITION DETERMINATION BY YFILER™ PLUS PCR AMPLIFICATION KIT FOR Y CHROMOSOME IDENTIFICATION

P. Iliev, B. Mirchev, E. Angelova, V. Djeliova, A. Hristov, Y. Kolev, M. Mileva, D. Krastev, A. Apostolov

Abstract: In expert practice, the Y chromosome analysis is an excellent method for detecting the presence of male DNA in mixed traces as well as for identifying males, or for establishing kinship. The goals of the research were to identify a minimal male component in mixed biological samples, as well as to identify unknown deceased men or to determine genealogical kinship. In the course of the research for identifying males with unconfirmed identity, with the help of Y chromosome markers, the rare allel 20.2 was detected at locus DYS627, embedded in the Yfiler™ Plus PCR Amplification Kit.
Keywords: material evidence, mixed biological traces, DNA identification, Y chromosome, rare allels.